Plant Synthetic Biology and Related (click on link to access paper)

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Tessema K. Kassaw, Alberto J. Donayre-Torres, Mauricio S. Antunes, Kevin J. Morey, and June I. Medford (2018) Engineering synthetic regulatory circuits in plants. Plant Science 273:13-22.

June I. Medford and Diane M. McCarthy (2017) Growing beyond: Designing plants to serve human and environmental interests. Current Opinion in Systems Biology 5:82-85.

Matthew J. Bick, Per J. Greisen, Kevin J. Morey, Mauricio S. Antunes, David La, Banumathi Sankaran, Luc Reymond, Kai Johnsson, June I. Medford, David Baker (2017) Computational design of environmental sensors for the potent opioid fentanyl. eLife 6:e28909.

Fredy Altpeter, Nathan M. Springer, Laura E. Bartley, Ann E. Blechl, Thomas P. Brutnell, Vitaly Citovsky, Liza J. Conrad, Stanton B. Gelvin, David P. Jackson, Albert P. Kausch, Peggy G. Lemaux, June I. Medford, Martha L. Orozco-Cárdenas, David M. Tricoli, Joyce Van Eck, Daniel F. Voytas, Virginia Walbot, Kan Wang, Zhanyuan J. Zhang and C. Neal Stewart Jr. (2016) Advancing crop transformation in the era of genome editing. The Plant Cell 28:1510-1520.

June Medford and Ashok Prasad (2016) Towards programmable plant genetic circuits. The Plant Journal 87:139-148.

Katherine A. Schaumberg, Mauricio S. Antunes, Tessema K. Kassaw, Wenlong Xu, Christopher S. Zalewski, June I. Medford & Ashok Prasad (2016) Quantitative characterization of genetic parts and circuits for plant synthetic biology. Nature Methods 13:94-100.

Justin Feng, Benjamin W Jester, Christine E Tinberg, Daniel J Mandell, Mauricio S Antunes, Raj Chari, Kevin J Morey, Xavier Rios, June I Medford, George M Church, Stanley Fields, & David Baker (2015) A general strategy to construct small molecule biosensors in eukaryotes. eLife 4:e10606.

June Medford and Ashok Prasad (2014) Plant synthetic biology takes root. Science 346:6206. | Article summary

Samanthe M. Lyons, Wenlong Xu, June I. Medford, and Ashok Prasad (2014) Loads bias genetic and signaling switches in synthetic and natural systems. PloS Computational Biology 10:3.

Mauricio S. Antunes, J. Jeff Smith, Derek Jantz, and June I. Medford (2012) Targeted DNA excision in Arabidopsis by a re-engineered homing endonuclease, BMC Biotechnology 12:86.

Kevin J. Morey, Mauricio S. Antunes, Matt J. Barrow, Fernando A. Solorzano, Keira L. Havens, J. Jeff Smith and June Medford (2012) Crosstalk between endogenous and synthetic components – synthetic signaling meets endogenous components, Biotechnology Journal 7 (7): 846-855.

Kevin J. Morey, Mauricio S. Antunes, Kirk D. Albrecht, Tessa A. Bowen, Jared F. Troupe, Keira L. Havens, June I. Medford (2011) Developing a Synthetic Signal Transduction System in Plants, In: Chris Voigt, Editor(s), Methods in Enzymology, Academic Press Volume 497, Synthetic Biology, Part A, Pages 581-602.

Antunes, M.S., Morey, K.J., Smith, J.J., Albrecht, K.D., Bowen, T.A., Zdunek, J.K., Troupe, J.F., Cuneo, M.J., Webb, C.T., Hellinga, H.W., and Medford, J.I. (2011). Programmable Ligand Detection System in Plants through a Synthetic Signal Transduction Pathway. PLoSOne 6, e16292

Mauricio S. Antunes, Kevin J. Morey, Neera Tewari-Singh, Tessa A. Bowen, J. Jeff Smith, Colleen T. Webb, Homme W. Hellinga, and June I. Medford (2009) Engineering Key Components in a Synthetic Eukaryotic Signal Transduction Pathway. Molecular and Systems Biology 5:270.

Bowen, Tessa A.  Zdunek, Jeffrey K. and June I. Medford (2008) Cultivating plant synthetic biology from systems biology.  New Phytologist 178:583-587.

Shaw, A.K., J.I. Medford, M. Antunes, W.S. McCormick, D. Wicker (2007)  Hyperspectral exploitation with plant sentinels. Proceedings of SPIE vol. 6554.  Chemical and Biological Sensing 7:6554-7.

Antunes, Mauricio S., Suk-Bong Ha, Neera Tewari-Singh, Kevin J. Morey, Anna M. Trofka, Paul Kugrens, Michael Deyholos, and June I. Medford (2006)  A synthetic degreening gene circuit provides a reporting system that is remotely detectable and has a reset capacity.  Plant Biotechnology Journal 4:605-622


Select Publications on Plant Development & Biotechnology

Parsons, Ronald L., Friederich J. Behringer and June I. Medford (2000) The SCHIZOID gene controls cell differentiation and cell division in Arabidopsis shoots. Planta  211:34-42.

Schultz, David, Edgar B. Cahoon, John Shanklin, Richard Craig, Diana L. Cox-Foster, Ralph O. Mumma, and June I. Medford  (1996) Expression of a Delta 9 14:0-ACP fatty acid desaturase gene is necessary for the production of w5 anacardic acids found in pest-resistant geranium (Pelargonium xhortorum ). Proceedings of the National Academy of Science 93: 8771-8775.

Cheryl L. Granger, Joseph D. Callos, and June I. Medford (1996).  Isolation of an Arabidopsis homologue of the maize homeobox KNOTTED 1 gene.  Plant Molecular Biology 31:373-378.

Long, Jeff A., Erich I. Moan, June. I. Medford, and M. Kathryn Barton (1996) The Arabidopsis SHOOTMERISTEMLESS gene encodes a member of the KNOTTED class of plant homeodomain proteins.  Nature 379: 66-69.

Callos, Joseph D., Marion D. Emerson, Bibo Xu, Bruce M. Link, Friederich Behringer and June Medford (1994). The FOREVER YOUNG gene encodes an oxidoreductase required for proper development of the Arabidopsis vegetative shoot apex.  The Plant Journal 6: 835-847.

Callos, Joseph D. and June I. Medford (1994). Organ Positions and Pattern Formation in the Shoot Apex. The Plant Journal 6:1-7.

Medford, June I., Joseph D. Callos, Friedrich J. Behringer and Bruce M. Link. (1994) Development of the vegetative shoot apical meristem. In: Arabidopsis. Elliot Meyerowitz and Chris Somerville, Eds. Cold Spring Harbor Press, Cold Spring Harbor, NY.  14:355-378.

Schultz, David, Richard Craig, Diana L. Cox-Foster, Ralph O. Mumma, and June I. Medford (1994)  RNA isolation from recalcitrant plant tissue.  Plant Molecular Biology Reporter 12: 310-316.

Medford, June I., Friedrich J. Behringer, Joseph D. Callos and Kenneth A. Feldmann (1992)  Normal and abnormal development of the vegetative shoot apex of Arabidopsis. The Plant Cell 4:631-643.

Behringer, Friedrich J. and June I. Medford (1992) A plasmid rescue technique for the recovery of plant DNA disrupted by T -DNA insertion. Plant Molecular Biology Reporter 10:60-

Medford, June I., J. Scott Elmer, Harry J. Klee (1991). Molecular cloning and characterization of genes expressed in shoot apical meristems. The Plant Cell 3:359-370.

Medford, June I. and Ian Sussex (1989). Regulation of chlorophyll and Rubisco accumulation in the embryonic cotyledons of Phaseolus vulgaris. Planta 179:309-315.

Medford, June I., Roger Horgan, Zaki El-Sawi, and Harry Klee (1989). Alterations of endogenous cytokinins in transgenic plants using a chimeric isopentenyl transferase gene.  The Plant Cell 1:403-413.

Hayford, Maria, June I. Medford, Nancy Hoffmann, Stephen Rogers, and Harry Klee (1988).  Development of a plant transformation selection system based on expression of genes encoding gentamicin acetyltransferases. Plant Physiology 86:1216-1222.