Recent Medford Lab Publications
Advancing crop transformation in the era of genome editing, The Plant Cell, July 2016.
Towards programmable plant genetic circuits, The Plant Journal, July 2016.
Quantitative characterization of genetic parts and circuits for plant synthetic biology, Nature Methods, January 2016.
A general strategy to construct small molecule biosensors in eukaryotes, eLife, December 2015.
June Medford and Ashok Prasad on the challenges and the future of Plant Synthetic Biology, in Science on 10 October 2014.
The Medford Lab is Hiring!
Our laboratory currently has openings for highly qualified, motivated people at the bachelor’s, master’s, and post-doc levels! Interested candidates for all positions should apply to the appropriate pool (Research Associate or Postdoctoral Fellow) at: http://www.biology.colostate.edu/employment/
The successful candidate must demonstrate U.S. employment eligibility. The Department of Biology will not provide visa sponsorship for these positions. There may be hiring restrictions given the federal funding sources.
Colorado State University is committed to providing an environment that is free from discrimination and harassment based on race, age, creed, color, religion, national origin or ancestry, sex, gender, disability, veteran status, genetic information, sexual orientation, gender identity or expression, or pregnancy. Colorado State University is an equal opportunity/equal access/affirmative action employer fully committed to achieving a diverse workforce and complies with all Federal and Colorado State laws, regulations, and executive orders regarding non-discrimination and affirmative action. The Office of Equal Opportunity is located in 101 Student Services.
Plant Synthetic Biology – What is it?
The Medford Lab at Colorado State University uses synthetic biology to redesign plants with useful traits and answer fundamental questions about natural processes. At its heart, synthetic biology differs from genetic engineering in its use of mathematical analysis, modeling, and orthogonal genetic parts to produce organisms with specialized functions. Synthetic biology in plants is a burgeoning field that shows great promise in areas such as bioenergy and security.
We have already developed a plant-based system with computer-designed receptors that activate a synthetic signal transduction pathway and trigger a transcriptional response. Using the tools of synthetic biology, we continue to adapt this system to new inputs and outputs. These outputs could include accumulation of biofuels, flowering or a visual response. At the same time, our investigations lead us to greater insights into how native molecules work. Our system is modular, allowing us to dissect fundamental plant processes. We are also developing digital genetic controls to allow precise regulation of our synthetic traits.
Medford Lab Projects
We have developed a genetic circuit that allows a plant to detect a substance and report on its presence in a way that people or cameras can see. The system has several components: a computer-designed protein custom designed to detect the substance, a synthetic “biological wire” pathway that moves the detection signal into the nucleus, and any one of a variety of readouts. Thanks to its highly modular design, this basic system can be adapted to a wide variety of uses including detecting environmental pollutants. You can read about our first Plant Sentinel, capable of detecting TNT, here.
…Enhancing Production of Bioenergy Crops & Their Controls
We are applying synthetic biology principles to bioenergy production. This work is supported by ARPA-E, the cutting edge program of the U.S. Department of Energy.
Many plant species with potential for use as bioenergy crops are currently under-utilized, due to slow traditional breeding programs and inefficient plant transformation methods. To speed up the development of plants with with enhanced bioenergy traits, we are engineering synthetic genetic switches with digital-like behavior that are capable of controlling development in these species. These switches will significantly increase the efficiency of transformation of these species.
…High-Throughput Testing Methodologies
Good parts make good synthetic circuits. To build a genetic circuit that has predictable function and produces an appropriate level of output, we are developing new protocols for testing large numbers of genetic parts rapidly and accurately. This will greatly expand the number of building blocks we can use as we develop new synthetic circuits for plants.
Joining the Medford Lab
Interested in joining our team? Apply to one of our Hiring Pools for Research Associates, Postdocs, and Research Scientists.